| Annotated protein: | Noelin-2 (Olfactomedin-2). Gene symbol: OLFM2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BM13 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ OLFM2 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645) |
| Synapse type(s): | cerebral cortex, glutamatergic retina, glutamatergic |
| Annotated paper: | Sultana A, et al. "Deletion of olfactomedin 2 induces changes in the AMPA receptor complex and impairs visual, olfactory, and motor functions in mice" Exp Neurol. 2014 Nov;261:802-11 PMID:25218043 |
| Figure(s): | 4, 6 |
| Annotation description: | Figure 4: Olfm2-KO mice Visual Evoked Potential (VEP) recording showed a significant reduction in the amplitude of the first negative wave (n1), the primary wave at the visual cortex evoked by an illuminative input to the eyes, in Olfm2-KOLacZ as compared with their WT littermates (Fig. 4A, B). Figure 6: Co-localization of Olfm2 protein with the AMPA receptor in the synaptosomal membrane fraction and its interaction with GluR2 authors conclude: "Olfm2, similar to Olfm1, interacted with the GluR2 subunit of the AMPAR complexes and Olfm2 co-segregated with the AMPA receptor subunit GluR2 and other synaptic proteins in the synaptosomal membrane fraction upon biochemical fractionation of adult mice cortex and retina. Immunoprecipitation from the synaptosomal membrane fraction of Olfm2 null mouse brain cortex using the GluR2 antibody showed reduced levels of several components of the AMPAR complex in the immunoprecipitates including Olfm1, PSD95 and CNIH2. These results suggest that heterodimers of Olfm1 and Olfm2 interact with AMPAR more efficiently than Olfm2 homodimers and that Olfm2 plays a role in the organization of the AMPA receptor complexes." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) IP + WB/MSMS Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "Noelins are 'universal' anchors controlling distribution and dynamics of AMPARs" and localized on PSD membranes @ PMID:37591201 |
| SynGO annotation ID: | 5532 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |