Annotated protein:Brorin (Brain-specific chordin-like protein) (CR (chordin-like cysteine-rich) domain-containing adhesive protein) (Cradin) (von Willebrand factor C domain-containing protein 2). Gene symbol: VWC2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8C8N3
antibody wiki:
SynGO gene info:SynGO data @ VWC2
Ontology domain:Biological Process
SynGO term:neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Boudkkazi S, et al. "A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors" Neuron. 2023 Aug 16;111(16):2544-2556.e9 PMID:37591201
Figure(s):4, 5, S3, S11
Annotation description:Figure 4,S3: AMPAR pulldowns (anti-GRIA1-4) yield Brorin (VWC2). In Noelin1-3(OLFM1-3) knockout mice, this interaction is lost.

Figure S11: in Brorin knockout mice, pulldowns against AMPAR yield reduced Noelin1 and Noelin2 levels (and no Brorin).

"Strikingly, the number of AMPARs detected by immuno-EM in the surface membrane was significantly reduced in both synapses and dendrites of Brorin-ablated neurons (Figure 5A), albeit to a lesser extent than by the removal of either Noe1-3 or Noe1 (Figure 2C), while the concomitant increase in AMPAR-free (silent) synapses was again about 2-fold (Figure 5A).

Similar to the Noelin KOs, the sEPSC amplitudes were decreased by the Brorin KO again with a stronger impact on INs than on principle cells as observed before (Figures 3A and 5B); the mEPSC amplitude was reduced by 23% compared with WT (Figure 5B).

Finally, Brorin-depleted CA1 PCs failed to effectively and robustly recruit additional AMPARs into the synapse in response to stimulation by the pairing-protocol (Figure 5C) although the observed impairment relative to WT was less pronounced than induced by removal of the Noelins (Figure 3)."
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:Whole-cell patch clamp
IP + WB/MSMS
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:5531
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology