| Annotated protein: | Brorin (Brain-specific chordin-like protein) (CR (chordin-like cysteine-rich) domain-containing adhesive protein) (Cradin) (von Willebrand factor C domain-containing protein 2). Gene symbol: VWC2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8C8N3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VWC2 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic membrane (GO:0098890) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Boudkkazi S, et al. "A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors" Neuron. 2023 Aug 16;111(16):2544-2556.e9 PMID:37591201 |
| Figure(s): | 4, 5, S3, S11 |
| Annotation description: | Figure 4,S3: AMPAR pulldowns (anti-GRIA1-4) yield Brorin (VWC2). In Noelin1-3(OLFM1-3) knockout mice, this interaction is lost. Figure S11: in Brorin knockout mice, pulldowns against AMPAR yield reduced Noelin1 and Noelin2 levels (and no Brorin). "Strikingly, the number of AMPARs detected by immuno-EM in the surface membrane was significantly reduced in both synapses and dendrites of Brorin-ablated neurons (Figure 5A), albeit to a lesser extent than by the removal of either Noe1-3 or Noe1 (Figure 2C), while the concomitant increase in AMPAR-free (silent) synapses was again about 2-fold (Figure 5A). Similar to the Noelin KOs, the sEPSC amplitudes were decreased by the Brorin KO again with a stronger impact on INs than on principle cells as observed before (Figures 3A and 5B); the mEPSC amplitude was reduced by 23% compared with WT (Figure 5B). Finally, Brorin-depleted CA1 PCs failed to effectively and robustly recruit additional AMPARs into the synapse in response to stimulation by the pairing-protocol (Figure 5C) although the observed impairment relative to WT was less pronounced than induced by removal of the Noelins (Figure 3)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5530 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |