| Annotated protein: | Clathrin light chain A (Lca). Gene symbol: CLTA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P08081 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CLTA |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Annotated paper: | Mueller VJ, et al. "Monitoring clathrin-mediated endocytosis during synaptic activity" J Neurosci. 2004 Feb 25;24(8):2004-12 PMID:14985443 |
| Figure(s): | Figures 1-6 |
| Annotation description: | "Several lines of evidence argue that the transient fluorescence increase of EGFP-LCa1 (clathrin light chain a, gene: CLTA) observed in synaptic boutons during stimulation reflects formation and disassembly of coated pits and is a direct measure of clathrin-mediated endocytosis in hippocampal synapses. First, EGFP-LCa1 inserts into structurally and functionally active coated pits in HEK cells (Fig. 1A,B). Second, reversible transient increases of fluorescence intensity are only observed during stimulation and are restricted to synaptic boutons. Adjacent axonal segments displayed small temporary decreases instead, such that the integrated fluorescence intensity along the axon remained nearly unchanged (Figs. 3D, 4A,B). Third, by estimating the local diffusion constant (between 0.13 and 0.44 μm2/sec) (Fig. 6D), we could show that this diffusional redistribution is sufficiently fast to be not rate-limiting, because most of the exchange occurs over a small distance (~1 μm), and the axon volume is large compared with the volume of the boutons. Fourth, if the signal was caused by a transient volume change, the same results should be obtained with overexpression of EGFP alone. Fifth, simulations based on a minimal model of LCa1 reaction and diffusion very faithfully predict the fluorescence changes observed in boutons and axons (Fig. 5B,C). We conclude that we were able to measure clathrin-mediated endocytosis in CNS synapses in real time." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Optical physiology Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5522 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |