| Annotated protein: | AP-2 complex subunit alpha-1 (100 kDa coated vesicle protein A) (Adaptor protein complex AP-2 subunit alpha-1) (Adaptor-related protein complex 2 subunit alpha-1) (Alpha-adaptin A) (Alpha1-adaptin) (Clathrin assembly protein complex 2 alpha-A large chain) (Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit). Gene symbol: AP2A1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P17426 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AP2A1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Annotated paper: | Lopez-Hernandez T, et al. "Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses" Elife. 2022 Jan 11;11:e71198 PMID:35014951 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3: In hippocampal neurons from AP-2u (gene Ap2m1) knockout mice, the ablated expression of the AP-2 complex was shown to result in defective endocytosis of VGLUT1-labeled synaptic vesicles (SV). While no comparable data is available for the alpha-adaptin subunits of the clathrin adaptor AP-2, it has been shown that; - AP2A is involved in SV endocytosis in C.elegans (PMID:23482940) and Drosophila (PMID:9118220) - AP2A1 and AP2A2 are both abundant in mammalian synaptic vesicles (PMID:33376223) Taken together, we here annotate AP2A1 as involved in synaptic vesicle endocytosis |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Optical physiology |
| Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Figure 7: α-adaptin (ap2-a) mutants exhibit weak efects in synaptic vesicle protein localization. Figure 8: Large vesicles accumulate at synapses in AP2 mutants. @ PMID:23482940 Figure 5: Drosophila-αAdaptin mutations impair the formation of endocytotic Synaptic Vesicles @ PMID:9118220 Dataset 1: AP2A1 and AP2A2 are among the top-most enriched synaptic vesicle proteins in a proteomic study of SV fractions prepared from rat brain @ PMID:33376223 structure of the 200 kDa AP2 "core" (α trunk, β2 trunk, μ2, and σ2) complexed with the polyphosphatidylinositol headgroup mimic inositolhexakisphosphate at 2.6 Å resolution. @ PMID:12086608 |
| SynGO annotation ID: | 5520 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |