| Annotated protein: | RNA-binding protein FUS (Protein pigpen). Gene symbol: FUS. Taxonomy: Mus musculus (Mouse). Uniprot ID: P56959 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FUS |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | hippocampus, glutamatergic cerebral cortex, glutamatergic hippocampus, GABAergic cerebral cortex, GABAergic |
| Annotated paper: | Sahadevan S, et al. "Synaptic FUS accumulation triggers early misregulation of synaptic RNAs in a mouse model of ALS" Nat Commun. 2021 May 21;12(1):3027 PMID:34021139 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1, S1: Confocal imaging of FUS in mouse cortex and hippocampus showed localization to along dendrites and axons as well as association with synaptic markers Synapsin-1 (SYN1) and PSD95 (DLG4). Figure 1c,d: sub-synaptic localization of FUS was determined using STED superresolution microscopy. Synaptic FUS preferentially associates with excitatory synapses, of which 46% contained FUS, while only 20% of analyzed inhibitory synapses showed FUS positivity. Figure 1h: The majority of FUS was found localized to the presynaptic terminal, approx 150nm from the active zone, but postsynaptic localization (away from the PSD) was also observed. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5514 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |