| Annotated protein: | Neurexin-2 (Neurexin II-alpha) (Neurexin-2-alpha). Gene symbol: NRXN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: E9Q7X7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NRXN2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Annotated paper: | Lin PY, et al. "Neurexin-2 restricts synapse numbers and restrains the presynaptic release probability by an alternative splicing-dependent mechanism" Proc Natl Acad Sci U S A. 2023 Mar 28;120(13):e2300363120 PMID:36961922 |
| Figure(s): | Figure 4 |
| Annotation description: | Nrxn2 is a transsynaptic adhesion molecule involved in synapse formation and specification (PMID:29100073). In absence of imaging data that confirms subcellular localization of Nrxn2 specifically (CC evidence and annotations are available for Nrxn-1 and -3), this annotations infers from functional studies on the role of Nrxn2 in synapse assembly (PMID:36608123) and pulldown assays that Nrxn2 is localized at the presynaptic active zone membrane. Figure 4: pulldowns of postsynaptic receptor proteins NL1B (neuroligin-1), NL3A2 (neuroligin-3) and LRRTM2 in mouse brain extracts yield neurexins 1, 2 and 3 (detection by SRM mass-spectrometry). This confirms the direct interaction of Nrxn2 with postsynaptic receptors. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | IP + WB/MSMS |
| Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Figure 1: "Constitutive deletion of Nrxn2 increases CA3 -> CA1 synaptic connections in the hippocampus." Figure 3: "The pan-neuronal deletion of Nrxn2 (Nrxn2 nKO) elevates CA3->CA1 synaptic connectivity and increases the release probability at CA3->CA1 synapses." Figure 4: "STORM superresolution microscopy reveals that the pan-neuronal Nrxn2 deletion (Nrxn2 nKO) increases the excitatory synapse density in the CA1 region." @ PMID:36608123 review of neurexins @ PMID:29100073 |
| SynGO annotation ID: | 5512 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |