| Annotated protein: | Synaptotagmin-1 (Synaptotagmin I) (SytI) (p65). Gene symbol: SYT1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P21707 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYT1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone (GO:0048786) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Hussain S, et al. "The calcium sensor synaptotagmin 1 is expressed and regulated in hippocampal postsynaptic spines" Hippocampus. 2017 Nov;27(11):1168-1177 PMID:28686803 |
| Figure(s): | 2 & 3 |
| Annotation description: | Figure 1D: Quantitative analysis of synaptotagmin 1 immunogold labeling over presynaptic terminal cytoplasm at synapses in the hippocampus of synaptotagmin 1 KO and WT mice. Figure 2A: Ultrastructural examination by immunogold EM show SYT1 localization to the presynaptic cytosol, presynaptic active zone, PSD, postsynaptic cytosol and the postsynaptic membrane (quantified in figure 3) Figure 2B: Ultrastructural examination by immunogold EM shows association of SYT1 to postsynaptic vesicles. Figure 2D-I: confirmation of postsynaptic localization of SYT1 with IF on dissociated hippocampal culture, co-staining with PSD95. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Microscopy (generic) |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5506 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |