Annotated protein:Synaptotagmin-1 (Synaptotagmin I) (SytI) (p65). Gene symbol: SYT1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P21707
antibody wiki:
SynGO gene info:SynGO data @ SYT1
Ontology domain:Cellular Component
SynGO term:presynaptic active zone (GO:0048786)
Synapse type(s):hippocampus
Annotated paper:Hussain S, et al. "The calcium sensor synaptotagmin 1 is expressed and regulated in hippocampal postsynaptic spines" Hippocampus. 2017 Nov;27(11):1168-1177 PMID:28686803
Figure(s):2 & 3
Annotation description:Figure 1D: Quantitative analysis of synaptotagmin 1 immunogold labeling over presynaptic terminal cytoplasm at synapses in the hippocampus of synaptotagmin 1 KO and WT mice.

Figure 2A: Ultrastructural examination by immunogold EM show SYT1 localization to the presynaptic cytosol, presynaptic active zone, PSD, postsynaptic cytosol and the postsynaptic membrane (quantified in figure 3)

Figure 2B: Ultrastructural examination by immunogold EM shows association of SYT1 to postsynaptic vesicles.

Figure 2D-I: confirmation of postsynaptic localization of SYT1 with IF on dissociated hippocampal culture, co-staining with PSD95.
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Microscopy (generic)
Annotator(s):Robin Clement
Guus Smit
Matthijs Verhage
ORCID:0000-0002-2286-1587
0000-0002-2514-0216
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:5506
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology