| Annotated protein: | Tyrosine-protein phosphatase non-receptor type 1 (EC 3.1.3.48) (Protein-tyrosine phosphatase 1B) (PTP-1B). Gene symbol: PTPN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P20417 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PTPN1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Fuentes F, et al. "Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning" PLoS One. 2012;7(7):e41536 PMID:22844492 |
| Figure(s): | Figures 3, 4 |
| Annotation description: | Figure 3: Effect of PTP1B (PTPN1) inhibition on dendritic protrusions of hippocampal neurons. (H) Quantification of the length of dendritic protrusions shows a significant increase in KO neurons compared to WT neurons. (I) Density of spines does not differ significantly between WT and KO. (J) Quantification of the different morphological types of spines reveals that KO neurons had a significantly reduced proportion of stubby and mushroom spines, and a significantly increased proportion of filopodia-like protrusions, compared to WT neurons. These results agree with those obtained in rat neurons expressing the dominant negative PTP1B(C/S). Figure 4: Analysis of DIV14 hippocampal neurons revealed that 63% of total spines in WT neurons, but only 31% of total spines in KO neurons, contained PSD-95. Authors conclude: "Here we found that impairing the expression of PTP1B in hippocampal neurons, by dominant negative or by gene targeting approaches, increased the proportion of filopodia-like protrusions, a hallmark of an immature stage of synaptogenesis, without affecting the density of total protrusions. Since dendritic protrusions are continuously appearing and disappearing [29,56,57], this result suggests that PTP1B is required for the normal maturation of dendritic spines and likely does not inhibit the turnover and genesis of protrusions" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5505 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |