| Annotated protein: | Small ubiquitin-related modifier 2 (SUMO-2) (SMT3 homolog 2) (Ubiquitin-like protein SMT3B) (Smt3B). Gene symbol: SUMO2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P61957 |
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| SynGO gene info: | SynGO data @ SUMO2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus, GABAergic Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Suk TR, et al. "Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain" iScience. 2023 Mar 9;26(4):106350 PMID:37009224 |
| Figure(s): | Figure 3, 4, S4 and S5 |
| Annotation description: | An HA-SUMO2 knock-in mouse model was used to assess the synaptic localisation of SUMO2. SUMO2 was detected via the HA antibody and WT animals were used as negative control for each experiments (immunofluorescence, western blot). SUMO2 localises at both the pre and post-synaptic compartments. "Quantitative analyses of anti-HA immunosignals confirmed a partial co-localization of Sumo2 with pre- and post-synaptic markers at excitatory and inhibitory synapses (Figure 3, Figure 4A, 3B, and and4A-4C).4A-4C)." SUMO2 is ubiquitin-like modifier that covalently modifies lysine residues of target proteins. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 5500 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |