Annotated protein:Serine/threonine-protein kinase ATR (EC 2.7.11.1) (Ataxia telangiectasia and Rad3-related protein). Gene symbol: ATR. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JKK8
antibody wiki:
SynGO gene info:SynGO data @ ATR
Ontology domain:Cellular Component
SynGO term:extrinsic component of synaptic vesicle membrane (GO:0098850)
Synapse type(s):cerebral cortex, GABAergic
Annotated paper:Cheng A, et al. "ATM and ATR play complementary roles in the behavior of excitatory and inhibitory vesicle populations" Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E292-E301 PMID:29279380
Figure(s):Figure 6, 7
Annotation description:Figure 5: confocal and super-resolution immunofluorescence imaging of ATR in 21DIV cultured cortical neurons showed co-localization with Clathrin and β-adaptin at synaptic puncta.

Figure 6, S5: super-resolution microscopy showed ATR co-localized with PSD95 (DLG4) and chromogranin-A (a DCV marker), but also in close proximity to Bassoon (BSN) in 21DIV cultured cortical neurons.

Figure 7, S6: super-resolution microscopy showed ATR co-localized with VGAT but not VGLUT in 21DIV cultured cortical neurons. ATR pulldowns yield VGAT but not VGLUT.

authors summarise;
"Taken together, these data strongly suggest that ATM is located in association with excitatory vesicles, while ATR is associated with inhibitory vesicles, consistent with their differential effects on the E/I balance in our cultures."
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Super resolution
Confocal
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:5495
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology