| Annotated protein: | Serine/threonine-protein kinase ATR (EC 2.7.11.1) (Ataxia telangiectasia and Rad3-related protein). Gene symbol: ATR. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JKK8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ATR |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of synaptic vesicle membrane (GO:0098850) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Cheng A, et al. "ATM and ATR play complementary roles in the behavior of excitatory and inhibitory vesicle populations" Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E292-E301 PMID:29279380 |
| Figure(s): | Figure 6, 7 |
| Annotation description: | Figure 5: confocal and super-resolution immunofluorescence imaging of ATR in 21DIV cultured cortical neurons showed co-localization with Clathrin and β-adaptin at synaptic puncta. Figure 6, S5: super-resolution microscopy showed ATR co-localized with PSD95 (DLG4) and chromogranin-A (a DCV marker), but also in close proximity to Bassoon (BSN) in 21DIV cultured cortical neurons. Figure 7, S6: super-resolution microscopy showed ATR co-localized with VGAT but not VGLUT in 21DIV cultured cortical neurons. ATR pulldowns yield VGAT but not VGLUT. authors summarise; "Taken together, these data strongly suggest that ATM is located in association with excitatory vesicles, while ATR is associated with inhibitory vesicles, consistent with their differential effects on the E/I balance in our cultures." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5495 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |