| Annotated protein: | Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAI-associated protein 2) (BAI1-associated protein 2) (Insulin receptor substrate protein of 53 kDa) (IRSp53) (Insulin receptor substrate p53) (Insulin receptor tyrosine kinase substrate protein p53). Gene symbol: BAIAP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6GMN2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ BAIAP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Choi J, et al. "Regulation of dendritic spine morphogenesis by insulin receptor substrate 53, a downstream effector of Rac1 and Cdc42 small GTPases" J Neurosci. 2005 Jan 26;25(4):869-79 PMID:15673667 |
| Figure(s): | fig 2/3/4 |
| Annotation description: | Immuno-EM localization of IRSp53 (BAIAP2) in the CA1 stratum radiatum of rat hippocampus. Numerous IRSp53 immunogold particles are associated with postsynaptic structures. Fig. 3 Colocalization of IRSp53 with PSD-95 in cultured neurons and synaptic localization of IRSp53 in brain sections. Shows colocalization of IRSp53 and PSD-95 in cultured hippocampal neurons (21 DIV). Furthermore crude synaptosomal extracts of adult rat brain immunoprecipitated with IRSp53 shows IRSp53 interaction with PSD-95 family in brain (Fig.2). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| Additional literature: | Cultured rat hippocampal neurons double stained with anti-IRSp58/53 and synaptophysin show IRSp53 presence at synapses (Fig 7). @ PMID:10460236 |
| SynGO annotation ID: | 549 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |