| Annotated protein: | Serine/threonine-protein kinase B-raf (EC 2.7.11.1) (Proto-oncogene B-Raf). Gene symbol: BRAF. Taxonomy: Mus musculus (Mouse). Uniprot ID: P28028 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ BRAF |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic modulation of chemical synaptic transmission (GO:0099170) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chen AP, et al. "Forebrain-specific knockout of B-raf kinase leads to deficits in hippocampal long-term potentiation, learning, and memory" J Neurosci Res. 2006 Jan;83(1):28-38 PMID:16342120 |
| Figure(s): | Figure 7 |
| Annotation description: | "e-LTP was tested with extracellular field recordings in stratum radiatum of hippocampal slices and was studied with a 2-theta-burst tetanus, because this pattern of stimulation is thought to reflect normal hippocampal patterns of activation during learning (Ranck, 1973; Otto et al., 1991). Our results demonstrate that e-LTP was deficient in the mutants." "The relationship between evoked fiber volleys and field EPSPs (fEPSPs) was indistinguishable between the Tg(αcre)B-raf-/- mutants (n = 12) and their B-raff/f controls (n = 13; Fig. 7A). Additionally, paired-pulse facilitation was also indistinguishable between the knockouts (n = 11) and the control mice (n = 12), suggesting that this forebrain- and neuron-specific knockout did not disrupt either synaptic transmission or presynaptic plasticity (Fig. 7B). Altogether, these results indicate that the conditional mutation of the B-raf gene does not compromise the general health of hippocampal slices, synaptic transmission, or certain measures of presynaptic plasticity." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Field recordings |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "our findings suggest that Rap1 suppresses neurotransmitter release through B-Raf-Erk signaling." @ PMID:23616533 |
| SynGO annotation ID: | 5483 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |