| Annotated protein: | Ribosomal protein S6 kinase alpha-1 (S6K-alpha-1) (EC 2.7.11.1) (90 kDa ribosomal protein S6 kinase 1) (p90-RSK 1) (p90RSK1) (p90S6K) (MAP kinase-activated protein kinase 1a) (MAPK-activated protein kinase 1a) (MAPKAP kinase 1a) (MAPKAPK-1a) (Ribosomal S6 kinase 1) (RSK-1). Gene symbol: RPS6KA1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63531 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RPS6KA1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic forebrain, glutamatergic |
| Annotated paper: | Suzuki T, et al. "Presence of up-stream and downstream components of a mitogen-activated protein kinase pathway in the PSD of the rat forebrain" Brain Res. 1999 Sep 4;840(1-2):36-44 PMID:10517950 |
| Figure(s): | Figure 1, 4 |
| Annotation description: | Figure 1: RSK1 (RPS6KA1) was enriched in all synaptic biochemical fractions prepared from rat forebrain, including PSD. Figure 4: electron microscopy of RSK1 in Rat hippocampus showed localization to the PSD. "RSK1 immunoreactive aggregations of 200-500 nm in diameter were scattered in the cytoplasm throughout the dendrites (arrows in Fig. 4). Some immunoreactive aggregates were in contact with, or very close to, the dendritic plasma membrane (arrows marked with 1 in Fig. 4a and b) or connected with immunoreactive PSDs (arrows marked with 2 in Fig. 4b) on the dendritic shaft. These results suggested that the immunoreactive aggregates observed in the central region of the dendrite cut longitudinally were close to the dendritic plasma membrane. Spines and spine PSDs were also strongly positive for RSK1 staining (Fig. 4c). Presynaptic terminals were negative for staining. The cytoplasmic region in the dendrites was relatively weak for staining, except for the immunoreactive aggregates." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5481 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |