| Annotated protein: | Alsin (Amyotrophic lateral sclerosis 2 protein homolog). Gene symbol: ALS2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q920R0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ALS2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Lai C, et al. "Amyotrophic lateral sclerosis 2-deficiency leads to neuronal degeneration in amyotrophic lateral sclerosis through altered AMPA receptor trafficking" J Neurosci. 2006 Nov 8;26(45):11798-806 PMID:17093100 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 1: Alsin (ALS2) interacts with GRIP1. Figure 2a: biochemical fractions prepared from mouse brain lysate showed ALS2 was enriched in PSD fractions, as were GRIP1, GluR2 (GRIA2), PSD95 (DLG4). Figure 2b: "GFP-tagged ALS2 and myc-tagged GRIP1 were cotransfected into cultured hippocampal neurons and immunostained with antibodies against GFP and myc, respectively. ALS2 (green) and GRIP1 (red) colocalized in the dendritic spines (arrows, bottom) as revealed by confocal microscopy. " |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | IP + WB/MSMS Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5472 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |