| Annotated protein: | ABI family, member 3. Gene symbol: ABI3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6AYC6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ABI3 |
| Ontology domain: | Biological Process |
| SynGO term: | modification of postsynaptic actin cytoskeleton (GO:0098885) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Bae J, et al. "NESH regulates dendritic spine morphology and synapse formation" PLoS One. 2012;7(4):e34677 PMID:22485184 |
| Figure(s): | Figures 2-8 |
| Annotation description: | Figure 2,3: Overexpression of NESH alters dendritic spine morphology. NESH knockdown causes abnormal morphological changes in dendritic spines. Figure 4: Overexpression of NESH prevents synapse formation in hippocampal neurons. Figure 5: NESH knockdown reduces synapse formation and affects the postsynaptic apparatus. Figure 6: NESH interacts directly with filamentous actin via its N-terminal region, but not with monomeric actin. Figure 7: Overexpression of NESH N-term inhibits spine maturation and synapse formation. Figure 8: NESH is involved in actin cytoskeleton rearrangement. |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Western blot IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "Interestingly, F-actin stabilization and disruption significantly affected the mobile fraction of NESH, possibly through altered interactions of NESH with the F-actin. In addition, NESH was synaptically targeted from the dendritic shaft to spine after induction of chemical LTP (long-term potentiation) and the translocation was dependent on F-actin. Our data collectively support the significance of the F-actin cytoskeleton in synaptic targeting of NESH as well as its dynamics." @ PMID:22496823 |
| SynGO annotation ID: | 5469 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |