| Annotated protein: | Serine/threonine-protein kinase PLK2 (EC 2.7.11.21) (Polo-like kinase 2) (PLK-2) (Serine/threonine-protein kinase SNK) (Serum-inducible kinase). Gene symbol: PLK2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9R012 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PLK2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation protein catabolic process at postsynapse (GO:0140252) |
| Annotated paper: | Pak DT, et al. "Targeted protein degradation and synapse remodeling by an inducible protein kinase" Science. 2003 Nov 21;302(5649):1368-73 PMID:14576440 |
| Figure(s): | Figure 1, 4 |
| Annotation description: | Figure 1-3: in COS-7 cells, SNK (PLK2) interacts with, and can degrate, postsynaptic protein SPAR (SIPA1L1). Further, SNK inhibits SPAR-mediated reorganization of actin cytoskeleton. Figure 4: immunofluorescence stainings in cultured hippocampal neurons showed overexpressed SNK is localized to dendritic spines and induces loss of SPAR, PSD-95 (DLG4), and Bassoon (BSN). Figure 5: overexpressed SNK induces loss of dendritic spines and increased filopodia in cultured hippocampal neurons. Figure 6: Synaptic activity induces endogenous SNK and leads to loss of SPAR and PSD-95. |
| Evidence tracking, Biological System: | Non-neuronal tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot IP + WB/MSMS Y2H |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "Plk2 directs elimination of Ras activator RasGRF1 and Rap inhibitor SPAR via phosphorylation-dependent ubiquitin-proteasome degradation. Conversely, Plk2 phosphorylation stimulates Ras inhibitor SynGAP and Rap activator PDZGEF1. These Ras/Rap regulators perform complementary functions to downregulate dendritic spines and AMPA receptors following elevated activity, and their collective regulation by Plk2 profoundly stimulates Rap and suppresses Ras. Furthermore, perturbation of Plk2 disrupts Ras and Rap signaling, prevents homeostatic shrinkage and loss of dendritic spines, and impairs proper memory formation." @ PMID:21382555 |
| SynGO annotation ID: | 5465 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |