| Annotated protein: | Serine/threonine-protein kinase PLK2 (EC 2.7.11.21) (Polo-like kinase 2) (PLK-2) (Serine/threonine-protein kinase SNK) (Serum-inducible kinase). Gene symbol: PLK2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9R012 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PLK2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Annotated paper: | Pak DT, et al. "Targeted protein degradation and synapse remodeling by an inducible protein kinase" Science. 2003 Nov 21;302(5649):1368-73 PMID:14576440 |
| Figure(s): | Figure 1, 4 |
| Annotation description: | Figure 1-3: in COS-7 cells, SNK (PLK2) interacts with, and can degrate, postsynaptic protein SPAR (SIPA1L1). Further, SNK inhibits SPAR-mediated reorganization of actin cytoskeleton. Figure 4: immunofluorescence stainings in cultured hippocampal neurons showed overexpressed SNK is localized to dendritic spines and induces loss of SPAR, PSD-95 (DLG4), and Bassoon (BSN). Figure 5: overexpressed SNK induces loss of dendritic spines and increased filopodia in cultured hippocampal neurons. Figure 6: Synaptic activity induces endogenous SNK and leads to loss of SPAR and PSD-95. |
| Evidence tracking, Biological System: | Non-neuronal tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot IP + WB/MSMS Y2H |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5464 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |