| Annotated protein: | Ras-specific guanine nucleotide-releasing factor 2 (Ras-GRF2) (Ras guanine nucleotide exchange factor 2). Gene symbol: RASGRF2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q99JE4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RASGRF2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Schwechter B, et al. "RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission" Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14462-7 PMID:23940355 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3: "RasGRF2 is present in spines. Hippocampal neurons were triple-labeled with antibodies against RasGRF2 and microtubule-associated protein 2 (Map2), and with phalloidin to visualize F-actin (abbreviated R, M, and P, and color-coded in merged images). Map2 and phalloidin primarily labels dendrites and spines, respectively. RasGRF2 staining is present in phalloidin-intense areas, indicating RasGRF2's presence in spines." Authors checked that their stainings did not yield signal from RasGRF1; "Because the RasGRF2 antibody we used cross-reacts with both isoforms of RasGRF (38), it was evident that RasGRF1 was not present in the cultured hippocampal neurons used for precipitation (input), although it was present in adult rat whole-brain lysate (in situ)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5462 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |