| Annotated protein: | Rap guanine nucleotide exchange factor 2 (Cyclic nucleotide ras GEF) (CNrasGEF) (Neural RAP guanine nucleotide exchange protein) (nRap GEP) (PDZ domain-containing guanine nucleotide exchange factor 1) (PDZ-GEF1) (RA-GEF-1) (Ras/Rap1-associating GEF-1). Gene symbol: RAPGEF2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8CHG7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAPGEF2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Mayanagi T, et al. "PSD-Zip70 Deficiency Causes Prefrontal Hypofunction Associated with Glutamatergic Synapse Maturation Defects by Dysregulation of Rap2 Activity" J Neurosci. 2015 Oct 21;35(42):14327-40 PMID:26490870 |
| Figure(s): | Figure 5, 6, 7 |
| Annotation description: | RapGEF2 (aka; PDZ-GEF1, nRap-GEP, CNrasGEF, RA-GEF) is a neural-specific activator for both mammalian Rap proteins Rap1 and Rap2 (PMID:10608883). Figure 5: reverse IPs showed RapGEF2 interacts with PSD-Zip70 (LZTS1). In cultured neurons, both proteins co-localized at dendritic puncta. PSD-Zip70 also interacted and co-localized with SPAR (SIPA1L1), which is known to be localized to the postsynaptic density (PMID:11502259). Figure 6: PSD-Zip70 modulates Rap2 activity via SPAR and RapGEF2 in cultured neurons. "Thus, the Rap2-specific activation in PSD-Zip70KO neurons may be related to the spatial association of PSD-Zip70 with SPAR and PDZ-GEF1 as a ternary complex close to Rap2 in the postsynapse." Figure 7: "We then investigated the effect of Rap2 activity on the spine morphology in WT and PSD-Zip70KO neurons. The forced expression of PDZ-GEF1 or PDZ-GEF2 reduced the size of the spine head and altered the spine shape from mushroom to thin in WT neurons (Fig. 7a-c)." |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | nRap-GEP (PDZGEF1/RapGEF2) interacts with Synaptic Scaffolding Molecule (S-SCAM/MAGI2), a postsynaptic scaffolding protein @ PMID:10548487 RapGEF2 (aka; PDZ-GEF1, nRap-GEP, CNrasGEF, RA-GEF) is a neural-specific activator for both mammalian Rap proteins Rap1 and Rap2 @ PMID:10608883 Silencing of RAPGEF2 prevents Aβ oligomer-induced spine loss @ PMID:33345400 |
| SynGO annotation ID: | 5458 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |