| Annotated protein: | Sodium/potassium/calcium exchanger 2 (Na(+)/K(+)/Ca(2+)-exchange protein 2) (Retinal cone Na-Ca+K exchanger) (Solute carrier family 24 member 2). Gene symbol: SLC24A2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BUN9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC24A2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Zhang Y, et al. "Anatomical evidence for a non-synaptic influence of the K+ -dependent Na+/Ca2+ -exchanger, NCKX2, on hippocampal plasticity" Neuroscience. 2015 Dec 3;310:372-88 PMID:26410028 |
| Figure(s): | Figure 13, 14 |
| Annotation description: | Figure 13, 14: After a series of low-resolution confocal imaging, authors used immunogold EM to confirm NCKX2 (SLC24A2) localization to the pre- and post-synapse. Localization to synaptic terminals was more prominent than localization to dendritic spines, authors note "Only a minority of gold particles were located on spines". Knockout mice were used as controls. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "The line profiles of NCKX2 (green) and FLAG-KIF21A (red) fluorescence intensities as well as the PDM image demonstrate that the NCKX2/FLAG-KIF21A complexes are localized in puncta along the axon fiber and in the axon terminal (Fig. 2G)." @ PMID:22442075 |
| SynGO annotation ID: | 5452 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |