| Annotated protein: | Kinesin-associated protein 3 (KAP-3) (KAP3). Gene symbol: KIFAP3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70188 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KIFAP3 |
| Ontology domain: | Biological Process |
| SynGO term: | anterograde dendritic transport of neurotransmitter receptor complex (GO:0098971) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Alsabban AH, et al. "Kinesin Kif3b mutation reduces NMDAR subunit NR2A trafficking and causes schizophrenia-like phenotypes in mice" EMBO J. 2020 Jan 2;39(1):e101090 PMID:31746486 |
| Figure(s): | Figure 2, 3, 7 |
| Annotation description: | The established heterotrimeric (KIF3A/KIF3B/KAP3) complex (KIF3 complex) is here shown to transport NR2 into dendritic spines (Fig. 7E). Figure 1: KIF3B binds to the NR2A complex and both interact with all KIF3 complex subunits as expected. Figure 2: KIF3B is essential for the postsynaptic localization of NR2A and NR2B in hippocampal neurons. Figure 3: KIF3B transports NR2A, but not NR2B. Figure 7: The expression of KIF3B, but not mutated KIF3B, restores spine morphology and NR2A surface expression. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | IP + WB/MSMS Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | biochemical characterization of KIF3A/B/C in rat brain. @ PMID:9487132 immunoprecipitations in mouse brain lysate show KIF3C associates with KIF3A, but not KIF3B. Also shows KIF3B/C in synaptic biochemical fractions and microtubule binding characteristics of KIF3C @ PMID:9450952 KIF3A/B are enriched in the same synaptic biochemical fractions prepared from mouse brain. KIF3A and KIF3B form a heterodimer in vitro that provides plus end--directed motor activity. The KIF3 complex Is a heterotrimeric motor in vivo. @ PMID:7559760 |
| SynGO annotation ID: | 5450 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |