| Annotated protein: | Leucine-rich repeat transmembrane neuronal protein 2 (Leucine-rich repeat neuronal 2 protein). Gene symbol: LRRTM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D4A7P2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRRTM2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | cerebellum, glutamatergic cerebral cortex, glutamatergic hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Linhoff MW, et al. "An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers" Neuron. 2009 Mar 12;61(5):734-49 PMID:19285470 |
| Figure(s): | Fig.3, Fig.7, Fig.S6 |
| Annotation description: | Fig. 2 LRRTM2 induces Excitatory Presynaptic Differentiation Literal "We tested whether the synaptogenic activity was restricted to excitatory or inhibitory presynaptic differentiation. The CFP fusion proteins were scored blind for significant clustering of the presynaptic vesicular neurotransmitter transporters,VGLUT1 andVGAT, to identify excitatory or inhibitory presynaptic differentiation, respectively." Fig. 7b Synaptic fractionation of adult rat brain homogenate shows LRRTM2 enrichment in the PSD fraction Literal "Synaptic fractionation of adult rat brain homogenate. The ~80 kDa band corresponding to LRRTM2 is abundant in the PSD fraction along with PSD-95. LRRTM2 is detected in the P2, synaptosome, and synaptic plasma membrane (SPM) fractions but less so in the cytoplasm and synaptic vesicle (SV) fractions. Fig. 7c-f Immunofluorescence data shows localization of LRRTM2 to glutamatergic postsynaptic sites in adult rodent brain. Literal "(C) LRRTM2 is widely detected in synaptic neuropil regions throughout the cortex and hippocampal formation. LRRTM2 exhibits a laminar-selective distribution; for example, in the CA1 region, LRRTM2 is present at higher levels in stratum lacunosum moleculare (SLM) than in radiatum (Rad) or oriens (Ori). (D) LRRTM2 is detected in the granule cell layer and molecular layer of the cerebellum. (C and D) Scale bar, 200 μm. (E) In hippocampus, LRRTM2 is particularly prominent in CA3 mossy fibers synapses shown here. Colocalization with the general synaptic marker bassoon and the excitatory synaptic marker VGLUT1 is shown. Enlarged regions are shown in the same color channels as the main panels (upper left LRRTM2 grayscale; lower left LRRTM2 green with bassoon blue; upper right LRRTM2 green with VGLUT1 red; lower right triple-channel overlay). (F) LRRTM2 also colocalized with bassoon and VGLUT1 in cerebellar glomerular synapses." 24/5/2017 Pim - Fig.3: non-neuronal cells transfected with LRRTM2-CFP (rat protein) and NMDAR (simulating a postsynaptic neuron) are able to induce presynaptic differentiation of a neuron (detectable glutamate release from SV's) - Fig.6: artificial clustering of over-expressed LRRTM2 in hipp neurons, induces post-synaptic differentiation. This data does not proove, but supports a postsynaptic localization of LRRTM4. - Fig.S6: in low density cultures over-expressed LRRTM2 trafficks into the dendrite and synaptic contacts. This also supports post-synaptic localization. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Biochemical fractionation (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 545 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |