| Annotated protein: | Kinesin-associated protein 3 (KAP-3) (KAP3). Gene symbol: KIFAP3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70188 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KIFAP3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Alsabban AH, et al. "Kinesin Kif3b mutation reduces NMDAR subunit NR2A trafficking and causes schizophrenia-like phenotypes in mice" EMBO J. 2020 Jan 2;39(1):e101090 PMID:31746486 |
| Figure(s): | Figure 1 |
| Annotation description: | The heterotrimeric (KIF3A/KIF3B/KAP3) complex (KIF3 complex) is here shown to transport NR2 into dendritic spines. Specifically KIF3B is shown to localize into the postsynapse by high resolution microscopy. There is no microscopy localization evidence for KAP3, but its localization to the postsynapse (as part of the KIF3 complex) is implied (Figure 7e). "Immunocytochemistry using cultured hippocampal neurons of Kif3b+/+ mice showed that KIF3B puncta were colocalized with APC and NR2A in dendritic shafts (Fig 1D) and in PSD95-positive spines (Fig 1E)." "Here, using mouse brain lysates, we found that NR2A was specifically immunoprecipitated by an anti-KIF3B antibody (Fig 1A), and KIF3A, KIF3B, and KAP3A were specifically immunoprecipitated by an anti-NR2A antibody (Fig 1B). These data suggested that KIF3A/KIF3B/KAP3 complex dominantly interacted with NR2A, rather than NR2B, proposing that KIF3B transports NR2A. We also found that KIF3B, KIF3A, KAP3A, NR2A, and PSD95 were all immunoprecipitated by an anti-APC antibody (Fig 1C), which suggested an interaction of these proteins with APC as a complex." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | IP + WB/MSMS Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | biochemical characterization of KIF3A/B/C in rat brain. @ PMID:9487132 immunoprecipitations in mouse brain lysate show KIF3C associates with KIF3A, but not KIF3B. Also shows KIF3B/C in synaptic biochemical fractions and microtubule binding characteristics of KIF3C @ PMID:9450952 KIF3A/B are enriched in the same synaptic biochemical fractions prepared from mouse brain. KIF3A and KIF3B form a heterodimer in vitro that provides plus end--directed motor activity. The KIF3 complex Is a heterotrimeric motor in vivo. @ PMID:7559760 |
| SynGO annotation ID: | 5448 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |