| Annotated protein: | Adenomatous polyposis coli protein (Protein APC) (mAPC). Gene symbol: APC. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61315 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ APC |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Alsabban AH, et al. "Kinesin Kif3b mutation reduces NMDAR subunit NR2A trafficking and causes schizophrenia-like phenotypes in mice" EMBO J. 2020 Jan 2;39(1):e101090 PMID:31746486 |
| Figure(s): | Figure 1 |
| Annotation description: | "Immunocytochemistry using cultured hippocampal neurons of Kif3b+/+ mice showed that KIF3B puncta were colocalized with APC and NR2A in dendritic shafts (Fig 1D) and in PSD95-positive spines (Fig 1E)." "Here, using mouse brain lysates, we found that NR2A was specifically immunoprecipitated by an anti-KIF3B antibody (Fig 1A), and KIF3A, KIF3B, and KAP3A were specifically immunoprecipitated by an anti-NR2A antibody (Fig 1B). These data suggested that KIF3A/KIF3B/KAP3 complex dominantly interacted with NR2A, rather than NR2B, proposing that KIF3B transports NR2A. We also found that KIF3B, KIF3A, KAP3A, NR2A, and PSD95 were all immunoprecipitated by an anti-APC antibody (Fig 1C), which suggested an interaction of these proteins with APC as a complex." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | IP + WB/MSMS Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5447 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |