| Annotated protein: | Kinesin-like protein KIF1A (Axonal transporter of synaptic vesicles). Gene symbol: KIF1A. Taxonomy: Mus musculus (Mouse). Uniprot ID: P33173 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KIF1A |
| Ontology domain: | Biological Process |
| SynGO term: | anterograde synaptic vesicle transport (GO:0048490) |
| Annotated paper: | Okada Y, et al. "The neuron-specific kinesin superfamily protein KIF1A is a unique monomeric motor for anterograde axonal transport of synaptic vesicle precursors" Cell. 1995 Jun 2;81(5):769-80 PMID:7539720 |
| Figure(s): | Figure 5 |
| Annotation description: | Figure 5: microscopic analyses showed motor protein KIF1A is involved in anterograde transport of SV precursors. "This sample was stained with anti-KIF1A antibody. As shown in Figure 5a, gradational staining was observed on the proximal side (left panel), becoming stronger in the regions closer to the ligated portions. In contrast, staining in regions distal to the ligated parts was significantly weaker (Figure 5a, right panel). In control samples, preabsorbed antibody supplemented with preimmune immunoglobulin G (IgG) was used as the primary antibody, and only much fainter background staining occurred. This means that the KIF1A in the axon is moving fast in the anterograde direction, strongly suggesting that it serves as a fast anterograde transporter. The lack of significant accumulation at the distal regions means that none or only very small fraction of KIF1A is transported retrogradely. Considering that KIFIA is not enriched in the synapse, KIF1A may be degraded there after traveling down the axon." "Which organelles are transported by KI F1A? To answer this question, we performed double staining of accumulated organelles at the proximal end of ligation. For this purpose, antibodies to several SV proteins (SV2, synaptotagmin, and synaptophysin) and a PM protein (syntaxin 1A) were used. All of them showed clear accumulation on the proximal side (Figures 5b-5d). No, or only weak, accumulation was observed on the distal side (data not shown). Thus, the major population of these proteins is demonstrated to be transported anterogradely." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | transport of SVs by KIF1A mutants in C.elegans @ PMID:31455732 characterization of KIF1A knockout mice @ PMID:9548721 KIF1A is required for Neurexin transport @ PMID:26446217 in C. elegans, UNC-104/KIF1A functions as an ARL-8 effector to regulate presynaptic cargo capture @ PMID:23727120 "Taken together, these data support a primary role for KIF1A in the anterograde transport of DCVs in mammalian neurons, and also provide evidence that KIF1A remains associated with DCVs during retrograde DCV transport." @ PMID:21256924 |
| SynGO annotation ID: | 5437 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |