| Annotated protein: | PTPRF interacting protein alpha 2. Gene symbol: PPFIA2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I6AM56 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA2 |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of postsynaptic density (GO:0098919) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Stucchi R, et al. "Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca(2+)/CaM Controls DCV Binding and Liprin-alpha/TANC2 Recruits DCVs to Postsynaptic Sites" Cell Rep. 2018 Jul 17;24(3):685-700 PMID:30021165 |
| Figure(s): | Figure 6, S4, S5 |
| Annotation description: | Figure 6: TANC2 and Liprin-α are scaffolding proteins in dendritic spines. Immunostaining of hippocampal neurons showed both proteins are localized to the PSD. Pulldowns confirmed both proteins have various PSD interactors. "Identifying TANC2 and liprin-α as enriched in dendritic spines led us to speculate that their distribution and accumulation may also be regulated by actin. Therefore, we transfected neurons with GFP-TANC2 or GFP-liprin-α2 and treated them with either latrunculin B to depolymerize the actin cytoskeleton or jasplakinolide to stabilize actin (Figures S4E and S4F). Latrunculin B treatment reduced the number of TANC2 and liprin-α2 clusters in spines by ~30%, whereas jasplakinolide treatment resulted in an opposite phenotype, with ~30% increase of clusters in spines (Figures S4G and S4H). These data suggest that the localization and clustering of TANC2 and liprin-α2 are affected by the actin cytoskeleton in dendritic spines. This hypothesis is strengthened by the ability of several TANC2- and liprin-α-interacting proteins to directly or indirectly associate with the actin cytoskeleton (Figure S4I)." Figure 6, S4: TANC2, KIF1A, and Liprin-α depletion affects spine density and morphology. "To get further insights into the roles of TANC2, KIF1A, and liprin-α in dendritic spine morphology, we performed knockdown experiments. TANC2 depletion caused a significant reduction in the total number of protrusions, particularly of mushroom spines (Figure 6F), and the effect was rescued by re-introducing full-length TANC2 (Figure 6F). KIF1A and liprin-α depletion showed a similar phenotype, severely affecting the total number of dendritic protrusions (Figure 6H). These data are consistent with previous findings (Han et al., 2010, McVicker et al., 2016) and show that TANC2, KIF1A, and liprin-α depletion affects spine number and morphology." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5436 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |