| Annotated protein: | PTPRF interacting protein alpha 2. Gene symbol: PPFIA2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I6AM56 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Stucchi R, et al. "Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca(2+)/CaM Controls DCV Binding and Liprin-alpha/TANC2 Recruits DCVs to Postsynaptic Sites" Cell Rep. 2018 Jul 17;24(3):685-700 PMID:30021165 |
| Figure(s): | Figure 6, S4 |
| Annotation description: | Figure 6, S4: immunofluorescence imaging of overexpressed liprin-α2 (PPFIA2) in hippocampal neurons showed enrichment in dendritic spines and strong co-localization with PSD marker proteins PSD95 (DLG4) and Homer. For liprin-α, the N-terminal part (1-735) is required for the synaptic localization (Figure S4B). Further, liprin-α2 pulldowns suggested interaction with various PSD proteins (as well as presynaptic active zone constituents). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5434 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |