| Annotated protein: | Kinesin-like protein KIF1A. Gene symbol: KIF1A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M4A4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KIF1A |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Stucchi R, et al. "Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca(2+)/CaM Controls DCV Binding and Liprin-alpha/TANC2 Recruits DCVs to Postsynaptic Sites" Cell Rep. 2018 Jul 17;24(3):685-700 PMID:30021165 |
| Figure(s): | Figure 7 |
| Annotation description: | Authors showed that motor protein KIF1A is involved in the transport of postsynaptic DCVs and in this process interacts with dendritic spine resident proteins. - KIF1A directly interacts with CaM and with the scaffolds liprin-α and TANC2. - KIF1A is regulated by a Ca2+/CaM-dependent mechanism, which allows for DCV loading. - Liprin-α and TANC2 are static PSD proteins that are not part of the KIF1A-DCV complex. - KIF1A-driven DCVs are recruited to dendritic spines by liprin-α and TANC2. "Co-expression of HA-KIF1A with GFP-TANC2 or GFP-liprin-α2 showed that ~10% KIF1A-FL co-clustered with TANC2 and liprin-α2 in dendritic spines (Figure 7A)." "We showed that calcium, acting via CaM, enhances KIF1A binding to DCVs and increases vesicle motility. In contrast, liprin-α and TANC2 are not part of the KIF1A-cargo complex but capture DCVs at dendritic spines." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5431 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |