| Annotated protein: | Myocardin related transcription factor A. Gene symbol: MRTFA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I6GFP9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MRTFA |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic structure (GO:0099159) |
| Synapse type(s): | cerebral cortex, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Kaneda M, et al. "Synaptic localisation of SRF coactivators, MKL1 and MKL2, and their role in dendritic spine morphology" Sci Rep. 2018 Jan 15;8(1):727 PMID:29335431 |
| Figure(s): | Figure 5, 6 |
| Annotation description: | Figure 5: shRNA knockdown of MKL1 (gene MRTFA) or MKL2 (gene MRTFB) in cultured cortical neurons resulted in a significant decrease in mushroom shaped dendritic spines (confocal imaging & morphology classification). The density of dendritic spines was not significantly affected. Figure 6: Double-knockout of MKL1 and MKL2 resulted in a more severe synapse maturation phenotype (stronger decrease in mushroom shaped synapses) and minor, but not statistically significant, reduction in spine density. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5426 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |