| Annotated protein: | Myocardin related transcription factor A. Gene symbol: MRTFA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I6GFP9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MRTFA |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Kaneda M, et al. "Synaptic localisation of SRF coactivators, MKL1 and MKL2, and their role in dendritic spine morphology" Sci Rep. 2018 Jan 15;8(1):727 PMID:29335431 |
| Figure(s): | Figure 3, 4 |
| Annotation description: | Figure 3: MKL1 (gene MRTFA) was detected in synaptic biochemical fractions prepared from rat brain homogenate. Although staining was faint, MKL1 appeared to be enriched in crude synaptic vesicle and PSD fractions as compared to P2 fraction. Figure 4: Immunostaining of mature cortical neuronal cultures showed endogenous MKL1 (gene MRTFA) was present in both presynapses (co-localization with synaptophysin/SYP) and dendritic spines (co-localization with PSD95/DLG4). There also seems to be ample non-synaptic staining and authors commented on this; "However, not all puncta stained with anti-MKL1 or anti-MKL2 antibodies colocalised with puncta stained with anti-PSD-95 or anti-synaptophysin antibodies." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5422 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |