| Annotated protein: | Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (Atrophin-1-interacting protein 1) (AIP-1) (Membrane-associated guanylate kinase inverted 2) (MAGI-2) (Synaptic-scaffolding molecule) (S-SCAM). Gene symbol: MAGI2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O88382 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MAGI2 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic membrane (GO:0098890) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Sumita K, et al. "Synaptic scaffolding molecule (S-SCAM) membrane-associated guanylate kinase with inverted organization (MAGI)-2 is associated with cell adhesion molecules at inhibitory synapses in rat hippocampal neurons" J Neurochem. 2007 Jan;100(1):154-66 PMID:17059560 |
| Figure(s): | Fig. 1, Fig. 8 |
| Annotation description: | Fig.1 MAGI2 is localized at inhibitory synapses. Literal "We found that 32.1 ± 5.4% of S-SCAM clusters were apposed to VGAT clusters, whereas 69.8 ± 5.8% of VGAT clusters were apposed to S-SCAM clusters. We also immunostained cells with anti-gephyrin antibody (Fig. 1b, lower panel). We found that 35.4 ± 7.4% of S-SCAM clusters were co-localized with gephyrin clusters, whereas 62.7 ± 10.9% of gephyrin clusters were co-localized with S-SCAM clusters. These findings suggest that almost one-third of signals detected by anti-S-SCAM antibody arelocalized at inhibitory synapses." Fig. 8e MAGI2 colocalized with neuroligin 2 at inhibitory synapses. Literal "In the immunofluorescence of rat primary cultured hippocampal neurons, neuroligin 2 was partially overlapped with S-SCAM and b-DG" 24/5/2017 Pim - Fig.1 shows that MAGI2 is localized at inh. synapses. - Fig.8a shows direct interaction of the cytosolic MAGI2 protein with transmembrane neurologin 2. - Fig.8e shows colocalization of MAGI2 and neuroligin 2 in inhibitory synapses. Neuroligin is a known postsynapic protein of inhibitory synapses that interacts with presynaptic neurexin proteins. In conclusion, via PPI and colocalization with neuroligin 2 a postsynaptic localization of MAGI2 is evidenced. Since MAGI2 interacts with the TM neuroligin protein the membrane association type is "extrinsic component...." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 542 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |