| Annotated protein: | Microtubule-associated protein 1B (MAP-1B) (MAP1(X)) (MAP1.2) [Cleaved into: MAP1B heavy chain; MAP1 light chain LC1]. Gene symbol: MAP1B. Taxonomy: Mus musculus (Mouse). Uniprot ID: P14873 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MAP1B |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tortosa E, et al. "Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation" J Biol Chem. 2011 Nov 25;286(47):40638-48 PMID:21984824 |
| Figure(s): | Figure 2 |
| Annotation description: | "MAP1B is present in some but not all dendritic spines. Dynamic MTs and MT-binding proteins have been recently detected in dendritic spines of mature neurons (32, 33, 34). Hence, we examined the detailed subcellular distribution of MAP1B in mature dendrites. Immunostaining of 21 DIV hippocampal neurons with antibodies directed against MAP1B (N19) and HMW-MAP2 (as a dendritic marker) revealed that MAP1B predominantly localized within the dendritic compartment (Fig. 2, A-D). However, immunostaining using anti-MAP1B (N19) and phalloidin (which targets F-actin and was used as a marker of dendritic spines and filopodia) demonstrated that MAP1B was occasionally observed in a small fraction of the dendritic protrusions (arrowhead in Fig. 2, E-G), colocalizing with F-actin (Fig. 2G). These F-actin-positive structures fulfilled the morphological and morphometric criteria to be considered dendritic spines. Immunostaining of MAP1B knock-out neurons was performed as a negative control (Fig. 2, H-J)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5416 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |