| Annotated protein: | Microtubule-associated protein 1A (MAP-1A) [Cleaved into: MAP1A heavy chain; MAP1 light chain LC2]. Gene symbol: MAP1A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P34926 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MAP1A |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone (GO:0048786) |
| Synapse type(s): | hippocampus cerebral cortex, glutamatergic |
| Annotated paper: | Leenders AG, et al. "The role of MAP1A light chain 2 in synaptic surface retention of Cav2.2 channels in hippocampal neurons" J Neurosci. 2008 Oct 29;28(44):11333-46 PMID:18971475 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 2a,b: LC2 (MAP1A light chain 2) is enriched in synaptosomal plasma membranes and (crude) synaptosomal fractions prepared from rat brain. LC2 was detected in PSD fractions, albeit at lower abundance than plasma membrane. MAP1A heavy chain was mostly expressed in the cytosolic fraction, like beta-tubulin and actin. Figure 2c: "Next, we sought to determine the distribution of LC2 at presynaptic terminals in more detail by immuno-electron microscopy of perfusion fixed brain tissue. LC2 was observed adjacent to the plasma membrane near or at the active zones or near the postsynaptic density" Figure 2d,e: Immunofluorescence imaging of 14DIV rat hippocampal neurons showed LC2 in punctate staining along neuronal processes and partially colocalized with synaptophysin (SYP). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5413 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |