| Annotated protein: | Phosphatase and actin regulator 1. Gene symbol: PHACTR1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62024 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PHACTR1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Allen PB, et al. "Phactrs 1-4: A family of protein phosphatase 1 and actin regulatory proteins" Proc Natl Acad Sci U S A. 2004 May 4;101(18):7187-92 PMID:15107502 |
| Figure(s): | Figure 5 |
| Annotation description: | Figure 5a: "In cultured hippocampal neurons transfected with an N-terminal GFP-tagged phactr construct, subcellular distribution was throughout cell bodies and dendrites, with apparent enrichment in dendritic spines. Phactr-1 was found in the perinuclear region but was absent from cell nuclei." Figure 5b: "Subcellular fractionation of rat brain homogenate supported the observation that phactr-1 may be localized at synapses because the protein was found to be present in postsynaptic density (PSD) fractions." |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5411 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |