| Annotated protein: | Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (Atrophin-1-interacting protein 1) (AIP-1) (Membrane-associated guanylate kinase inverted 2) (MAGI-2) (Synaptic-scaffolding molecule) (S-SCAM). Gene symbol: MAGI2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O88382 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MAGI2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Hirao K, et al. "A novel multiple PDZ domain-containing molecule interacting with N-methyl-D-aspartate receptors and neuronal cell adhesion proteins" J Biol Chem. 1998 Aug 14;273(33):21105-10 PMID:9694864 |
| Figure(s): | Fig.6, Fig.7, Fig.8 |
| Annotation description: | Fig. 6 MAGI2 interacts with NMDA Literal "The anti-S-SCAM antibody coimmunoprecipitated NMDAR1, whereas the preimmune serum did not.This result indicates that S-SCAM interacts with NMDA receptors in vivo." Fig. 7 MAGI2 colocalyzed with PSD-95 Literal "To examine the distribution of S-SCAM in neurons, we double-stained primary cultured rat hippocampal neurons on day 14 in the combination of anti-S-SCAM antibody with either anti-NMDAR1 or anti-PSD- 95/SAP90 antibody. The staining pattern of S-SCAM was similar to that of PSD-95/SAP90, which was detected in the cell body as well as at the dendrites (Fig. 7A). S-SCAM showed distributions similar to those of NMDAR1 at dendrites (Fig.7B). Because SAPAP was distributed in a manner similar to that of PSD-95/SAP90 (Fig. 7C), S-SCAM was considered to be distributed in a manner similar to SAPAP. Fig. 8 Literal "We also determined the distribution of S-SCAM in the subcellular fractions of rat brain by the immunoblotting with the anti-S-SCAM antibody. The signals detected with the antibody were distributed in both the cytosolic synaptosomal (S2) and crude synaptosomal pellet (P2) fractions (Fig. 8). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Protein-protein interaction (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 541 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |