| Annotated protein: | Alpha-adducin (Erythrocyte adducin subunit alpha). Gene symbol: ADD1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9QYC0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADD1 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | hippocampus |
| Annotated paper: | O'Neil SD, et al. "Action potential-coupled Rho GTPase signaling drives presynaptic plasticity" Elife. 2021 Jul 16;10:e63756 PMID:34269176 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 1: Add1 was detected in proximity labeling of synapsin using BioID. Figure 2: Cultured hippocampal neurons were infected on DIV0 with AAVs containing the candidate sgRNA and a 2x-HA-V5-Myc HiUGE donor in the corresponding open reading frame. Confocal imaging showed Add1 co-localized with synapsin 1, but the resolution is too low to discern localization to the pre/post-synapse. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Mass-spectrometry |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5401 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |