| Annotated protein: | Potassium channel subfamily T member 1. Gene symbol: KCNT1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6ZPR4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCNT1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane potential (GO:0060078) |
| Synapse type(s): | forebrain |
| Annotated paper: | Matt L, et al. "The Na(+)-activated K(+) channel Slack contributes to synaptic development and plasticity" Cell Mol Life Sci. 2021 Dec;78(23):7569-7587 PMID:34664085 |
| Figure(s): | Figure 2-5 |
| Annotation description: | Figure 2: in biochemical fractions prepared from mice forebrain, Slack (KCTN1) was enriched in PSD fractions. Staining was abolished in Slack knockout mice. Figure 2, 3: Infant Slack-/- show reduced NMDAR-mediated transmission and lack mGluR LTD. Figure 4: Slack-/- deficiency impairs dephosphorylation of GluA1 S845 after LTD induction. Figure 5: NMDAR-dependent LTD but not LTP is impaired in adult Slack-/-. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "Combined, our findings suggest that Slack-dependent K+ signals oppose the NMDAR-mediated excitotoxic neuronal injury by promoting pro-survival signaling via the BDNF/TrkB and Erk axis." @ PMID:33817875 The sodium-activated potassium channels Slick (Slo2.1, KCNT2) and Slack (Slo2.2, KCNT1) are paralogous channels of the Slo family of high-conductance potassium channels. - Slick and Slack channels co-localize in the same cellular compartment. - Slick and Slack channels assemble into cellular protein complexes in mouse brain. - potential interaction partners include TMEM293, SNCB, DPP10, DLG3 @ PMID:29124216 "Our findings suggest that Slack channels regulate synaptic transmission within the spinal cord dorsal horn and by doing so establishes the threshold for thermal nociception." @ PMID:28604221 "Large-conductance calcium-activated potassium channels (maxi-K channels) have an essential role in the control of excitability and secretion. Only one gene Slo is known to encode maxi-K channels, which are sensitive to both membrane potential and intracellular calcium. We have isolated a potassium channel gene called Slack that is abundantly expressed in the nervous system. Slack channels rectify outwardly with a unitary conductance of about 25-65 pS and are inhibited by intracellular calcium. However, when Slack is co-expressed with Slo, channels with pharmacological properties and single-channel conductances that do not match either Slack or Slo are formed. The Slack/Slo channels have intermediate conductances of about 60-180 pS and are activated by cytoplasmic calcium. Our findings indicate that some intermediate-conductance channels in the nervous system may result from an interaction between Slack and Slo channel subunits." @ PMID:10196543 |
| SynGO annotation ID: | 5400 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |