| Annotated protein: | Sphingosine 1-phosphate receptor 2 (S1P receptor 2) (S1P2) (Endothelial differentiation G-protein coupled receptor 5) (Lysophospholipid receptor B2) (Sphingosine 1-phosphate receptor Edg-5) (S1P receptor Edg-5). Gene symbol: S1PR2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P52592 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ S1PR2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Skoug C, et al. "Sphingosine 1-Phoshpate Receptors are Located in Synapses and Control Spontaneous Activity of Mouse Neurons in Culture" Neurochem Res. 2022 Oct;47(10):3114-3125 PMID:35781853 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1: S1PR2 was detected in synaptosomal biochemical fractions prepared from mouse cortex, with slight enrichment in the synaptosome-subfraction enriches the active zone (membrane) as compared to synaptosomes. S1PR2 was also detected in the postsynaptic fraction, albeit at slightly lower level than synaptosomes (and much lower than active zone fraction). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | low-resolution imaging showed S1PR1 (S1P1 receptor) co-localizes with synaptophysin in rat hippocampal slices. Not high resolution enough to establish presynaptic localization by itself, but follow-up electrophysiology of SphK1 knockouts suggest a presynaptic phenotype which subsequently makes it plausible that Sphingosine 1-phosphate receptors are present presynaptically @ PMID:20950672 Figure 2: Nogo-A (gene RTN4) binds to S1PR2. Figure 3: S1PR2 is internalized upon Nogo-A-Δ20 binding in 3T3 cells. Figure 5: Nogo-A-Δ20 inhibition is mediated via the G13-LARG-RhoA signaling axis and can be modulated by exogenous S1P in 3T3 cells. Figure 6: Blockade of S1PR2 phenocopies the increase in hippocampal and cortical LTP observed upon Nogo-A neutralization. @ PMID:24453941 |
| SynGO annotation ID: | 5396 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |