Annotated protein:	Reticulon-4 (Neurite outgrowth inhibitor) (Nogo protein). Gene symbol: RTN4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99P72-2
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SynGO gene info:	SynGO data @ RTN4
Ontology domain:	Biological Process
SynGO term:	regulation of postsynapse assembly (GO:0150052)
Synapse type(s):	hippocampus, glutamatergic
Annotated paper:	Kellner Y, et al. "Nogo-A controls structural plasticity at dendritic spines by rapidly modulating actin dynamics" Hippocampus. 2016 Jun;26(6):816-31 PMID:26748478
Figure(s):	Figure 1, 2, 3
Annotation description:	Figure 1-3: Nogo-A (gene RTN4) inhibition through a specific antibody, as well as inhibition of its receptors NgR (gene RTN4R) and S1PR2, affects dendritic spine development in CA3 pyramidal neurons. Confocal imaging after application showed spine density and morphology was significantly affected. This was confirmed by FRAP of eGFP-actin in spines.
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Antagonist / agonist
Evidence tracking, Experiment Assay:	Fluorescence recovery after photobleaching (FRAP) Confocal
Annotator(s):	Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
Additional literature:	Figure 2: Nogo-A (gene RTN4) binds to S1PR2. Figure 3: S1PR2 is internalized upon Nogo-A-Δ20 binding in 3T3 cells. Figure 5: Nogo-A-Δ20 inhibition is mediated via the G13-LARG-RhoA signaling axis and can be modulated by exogenous S1P in 3T3 cells. Figure 6: Blockade of S1PR2 phenocopies the increase in hippocampal and cortical LTP observed upon Nogo-A neutralization. @ PMID:24453941
SynGO annotation ID:	5391
Dataset release (version):	20231201
View annotation as GO-CAM model: