| Annotated protein: | Sphingosine 1-phosphate receptor 2 (S1P receptor 2) (S1P2) (Endothelial differentiation G-protein coupled receptor 5) (Lysophospholipid receptor B2) (Sphingosine 1-phosphate receptor Edg-5) (S1P receptor Edg-5). Gene symbol: S1PR2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P52592 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ S1PR2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kellner Y, et al. "Nogo-A controls structural plasticity at dendritic spines by rapidly modulating actin dynamics" Hippocampus. 2016 Jun;26(6):816-31 PMID:26748478 |
| Figure(s): | Figure 1, 2, 3 |
| Annotation description: | Figure 1-3: using a specific blocker (JTE-013) for the sphingosine-1-phosphate receptor 2 (S1PR2) affects dendritic spine development in CA3 pyramidal neurons. Confocal imaging after application showed spine density and morphology was significantly affected. This was confirmed by FRAP of eGFP-actin in spines. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Fluorescence recovery after photobleaching (FRAP) Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | in biochemical fractions from mouse cortex, S1PR2 is enriched in the fraction that holds presynaptic membrane. This prep possibly contains postsynaptic membranes as well. Further, S1PR2 was detected in postsynaptic fractions albeit at lower abundance. @ PMID:35781853 Figure 2: Nogo-A (gene RTN4) binds to S1PR2. Figure 3: S1PR2 is internalized upon Nogo-A-Δ20 binding in 3T3 cells. Figure 5: Nogo-A-Δ20 inhibition is mediated via the G13-LARG-RhoA signaling axis and can be modulated by exogenous S1P in 3T3 cells. Figure 6: Blockade of S1PR2 phenocopies the increase in hippocampal and cortical LTP observed upon Nogo-A neutralization. @ PMID:24453941 |
| SynGO annotation ID: | 5390 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |