| Annotated protein: | Destrin (Actin-depolymerizing factor) (ADF) (Sid 23). Gene symbol: DSTN. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9R0P5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DSTN |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Gorlich A, et al. "N-cofilin can compensate for the loss of ADF in excitatory synapses" PLoS One. 2011;6(10):e26789 PMID:22046357 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1c-f: confocal imaging of immunofluorescence labeling for ADF (Actin-depolymerizing factor, gene DSTN), Synaptophysin (SYP) and PSD-95 (DLG4) in cultured hippocampal neurons showed localization to both pre- and post-synapse. Figure 1g-i: immunogold EM of ADF in mouse CA1 and CA3 stratum radiatum showed localization to both pre- and post-synapse, with higher abundance in the former. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5376 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |