| Annotated protein: | Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAI-associated protein 2) (BAI1-associated protein 2) (Insulin receptor substrate protein of 53 kDa) (IRSp53) (Insulin receptor substrate p53) (Insulin receptor tyrosine kinase substrate protein p53). Gene symbol: BAIAP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6GMN2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ BAIAP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chazeau A, et al. "Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion" EMBO J. 2014 Dec 1;33(23):2745-64 PMID:25293574 |
| Figure(s): | Figure 5 |
| Annotation description: | Figure 5: overexpression of mEOS2-IRSp53 (gene BAIAP2) in dissociated hippocampal neurons followed by super-resolution imaging showed localization to dendritic spine heads. "In spines, IRSp53 displayed almost the same dynamics and sub-spine localization than WAVE complex subunits. Many confined trajectories formed a long-lasting domain of IRSp53 co-localizing with the PSD (Supplementary Figs S6 and S9C). In addition, mEOS2-IRSp53 displayed diffusion properties similar to mEOS2-Abi1 and mEOS2-Nap1 (Fig (Fig5G-I,5G-I, Supplementary Fig S5E-G), indicating that IRSp53 is confined in the same membrane domain. LatA treatment did not induce loss of Abi1 or IRSp53 accumulation in spines, which remained associated with the PSD (Supplementary Fig S7C and D), demonstrating that their recruitment in spines is not mediated by interactions with F-actin networks." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5375 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |