| Annotated protein: | Formin-like 2. Gene symbol: FMNL2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I6AV50 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FMNL2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chazeau A, et al. "Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion" EMBO J. 2014 Dec 1;33(23):2745-64 PMID:25293574 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 2b: overexpression of mEOS2-FMNL2 in dissociated hippocampal neurons followed by super-resolution imaging showed localization to dendritic spine heads. "Live super-resolution intensity images showed that mEOS2-VASP and FMNL2-mEOS2 accumulated at the tips of the finger-like extensions in spines (Fig2B and C). Time-lapse recordings demonstrated that those extensions corresponded to protrusions, lost after CD treatment, and moving forward with velocities close to the ones measured for lamellipodia and filopodia (~60 nm s-1; 22 protrusions) (Svitkina et al, 2003; Giannone et al, 2004) (Supplementary Fig S2). We used direct stochastic optical reconstruction microscopy (dSTORM) (Van de Linde et al, 2011) and demonstrated that endogenous VASP and FMNL2 were also enriched at the tips of finger-like protrusions in spines (Supplementary Fig S1B and C). Thus, spine motility is supported by multiple finger-like protrusions driven by the polarized elongation of F-actin barbed ends." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5372 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |