| Annotated protein: | Actin-related protein 2/3 complex subunit 2 (Arp2/3 complex 34 kDa subunit) (p34-ARC). Gene symbol: ARPC2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9CVB6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARPC2 |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic vesicle membrane (GO:0030672) |
| Synapse type(s): | hippocampus |
| Annotated paper: | O'Neil SD, et al. "Action potential-coupled Rho GTPase signaling drives presynaptic plasticity" Elife. 2021 Jul 16;10:e63756 PMID:34269176 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3: immunogold EM imaging of ArpC2 (aka. Arp2/3) in mouse CA1 hippocampus showed localization to synaptic vesicle membranes and pre/postsynapse (distant from the plasma membrane and release sites). "ArpC2 was concentrated in dendritic spines approximately 200 nm below the PSD (Figure 3F). However, a fraction of gold particles (~27%) localized to presynaptic terminals with a consistent and specific distribution. ArpC2 was overlapping with synaptic vesicles, but it also localized to the presynaptic area beyond the synaptic vesicle cluster, away from the active zone (mean distance ± SD, 298 ± 159 nm for ArpC2, and 173 ± 129 nm for synaptic vesicles). Very little immunolabeling was observed when the primary antibody was omitted as a negative control. In the few synapses that did have staining (<1%), there was diffuse non-specific signal across the synapse (Figure 3-figure supplement 1D-E). Taken together, the overlapping distributions of Rac1 and Arp2/3 at synaptic vesicles suggest a potential common presynaptic function related to synaptic vesicle modulation." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5365 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |