| Annotated protein: | Ubiquitin-conjugating enzyme E2 N (EC 2.3.2.23) (Bendless-like ubiquitin-conjugating enzyme) (E2 ubiquitin-conjugating enzyme N) (Ubiquitin carrier protein N) (Ubiquitin-protein ligase N). Gene symbol: UBE2N. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9EQX9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ UBE2N |
| Ontology domain: | Biological Process |
| SynGO term: | regulation protein catabolic process at postsynapse (GO:0140252) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ma Q, et al. "Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity" Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8760-E8769 PMID:28973854 |
| Figure(s): | Figure 3 |
| Annotation description: | "In vitro ubiquitination assays using purified proteins showed that TRAF6, in conjunction with Ubc13/Uev1A, effectively delivered the ubiquitin moieties to PSD-95, which did not occur when TRAF6, UbK63, Ubc13, Uev1A, or PSD-95 was omitted from the reaction mix (Fig. 3E)." "Interactions between endogenous PSD-95 and TRAF6, Ubc13, Uev1A, or CYLD in mouse forebrain were also detected (Fig. S3 F-I)." "The activity-dependent assembly/disassembly of K63-polyUb chains may involve dynamic regulation of the E2-E3-substrate-DUB multiprotein complex we have identified in the PSD. The Ubc13/Uev1A dimer remains the only known K63-selective E2 that, together with the RING-domain family E3 ligase TRAF6, catalyzes the assembly of K63-polyUb chains on substrates. Our results suggest that the Ubc13/Uev1A-TRAF6 complex binds the PSD-95 NT and delivers ubiquitin moieties to cognate lysine sites." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5359 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |