| Annotated protein: | TNF receptor-associated factor 6 (EC 2.3.2.27) (E3 ubiquitin-protein ligase TRAF6) (RING-type E3 ubiquitin transferase TRAF6). Gene symbol: TRAF6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B5DF45 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TRAF6 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation protein catabolic process at postsynapse (GO:0140252) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ma Q, et al. "Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity" Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8760-E8769 PMID:28973854 |
| Figure(s): | Figure 3, 7 |
| Annotation description: | "TRAF6-facilitated K63 ubiquitination was diminished in K558R (Fig. S3B) and PSD-95ΔNT (Fig. 3D) mutants. In vitro ubiquitination assays using purified proteins showed that TRAF6, in conjunction with Ubc13/Uev1A, effectively delivered the ubiquitin moieties to PSD-95, which did not occur when TRAF6, UbK63, Ubc13, Uev1A, or PSD-95 was omitted from the reaction mix (Fig. 3E)." "Knocking down TRAF6 prevented the AP5-elicited increase of PSD-95 ubiquitination (Fig. 7G), supporting the notion that TRAF6 mediates constitutive K63 ubiquitination of PSD-95." "The activity-dependent assembly/disassembly of K63-polyUb chains may involve dynamic regulation of the E2-E3-substrate-DUB multiprotein complex we have identified in the PSD. The Ubc13/Uev1A dimer remains the only known K63-selective E2 that, together with the RING-domain family E3 ligase TRAF6, catalyzes the assembly of K63-polyUb chains on substrates. Our results suggest that the Ubc13/Uev1A-TRAF6 complex binds the PSD-95 NT and delivers ubiquitin moieties to cognate lysine sites." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5358 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |