| Annotated protein: | Ubiquitin conjugating enzyme E2 V1. Gene symbol: UBE2V1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZFY8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ UBE2V1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ma Q, et al. "Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity" Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8760-E8769 PMID:28973854 |
| Figure(s): | Figure 3, S3 |
| Annotation description: | Figure 3: in biochemical fractions prepared from rat forebrain, Uev1A (UBE2V1) was enriched in PSD fractions. Figure S3: confocal imaging of immunofluorescence labeling for Uev1A and PSD-95 (DLG4) in cultured hippocampal neurons showed partial co-localization. Further, Uev1A immunoprecipitation in mouse forebrain yields PSD95. "In vitro ubiquitination assays using purified proteins showed that TRAF6, in conjunction with Ubc13/Uev1A, effectively delivered the ubiquitin moieties to PSD-95, which did not occur when TRAF6, UbK63, Ubc13, Uev1A, or PSD-95 was omitted from the reaction mix (Fig. 3E)." |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) IP + WB/MSMS Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5357 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |