| Annotated protein: | Ubiquitin-conjugating enzyme E2 N (EC 2.3.2.23) (Bendless-like ubiquitin-conjugating enzyme) (E2 ubiquitin-conjugating enzyme N) (Ubiquitin carrier protein N) (Ubiquitin-protein ligase N). Gene symbol: UBE2N. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9EQX9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ UBE2N |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ma Q, et al. "Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity" Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8760-E8769 PMID:28973854 |
| Figure(s): | Figure 3, S3 |
| Annotation description: | Figure S3: confocal imaging of immunofluorescence labeling for Ubc13 (UBE2N) and PSD-95 (DLG4) in cultured hippocampal neurons showed Ubc13 localization is ubiquitous in dendrites and spines, and partially co-localizes with PSD95 (in biochemical fractions, Ubc13 was also ubiquitous. Fig. 3). Further, Ubc13 immunoprecipitation in mouse forebrain yields PSD95. "In vitro ubiquitination assays using purified proteins showed that TRAF6, in conjunction with Ubc13/Uev1A, effectively delivered the ubiquitin moieties to PSD-95, which did not occur when TRAF6, UbK63, Ubc13, Uev1A, or PSD-95 was omitted from the reaction mix (Fig. 3E)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) IP + WB/MSMS Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5356 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |