| Annotated protein: | Caskin-1 (CASK-interacting protein 1). Gene symbol: CASKIN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6P9K8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CASKIN1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic density assembly (GO:0099151) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Bencsik N, et al. "Dendritic spine morphology and memory formation depend on postsynaptic Caskin proteins" Sci Rep. 2019 Nov 14;9(1):16843 PMID:31727973 |
| Figure(s): | Figure 5 |
| Annotation description: | "Average protrusion density in Caskin dKO neurons transfected only with EGFP was significantly reduced compared to EGFP-expressing WT neurons but was not influenced by Caskin1 overexpression (Fig. 5A). On the other hand, when morphological parameters of the protrusions were analysed in more detail (see Fig. 5B-D, plotting the length along the head-to-neck ratio [HN-index] of the individual protrusions24); the density of mushroom-like protrusions in Caskin dKO neurons was significantly lower than in wild-type neurons. Overexpression of Caskin1 in Caskin dKO neurons increased significantly the ratio of mushroom-like protrusions in expense for thin, filopodial-like protrusions (Fig. 5A)." "Taken together, our data indicate that lack of endogenous Caskin proteins leads to reduced PSD length and shrinkage of dendritic spines in vivo and decreased protrusion density in vitro, while overexpression of Caskin1 leads to the expansion of dendritic spine heads in cultivated neurons within 24 hours following transfection." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5355 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |